16- or 24-channel linear electrode arrays with 50 μm interelectrode spacing v-probes  (plexon inc)

Journal: iScience

Article Title: Global and local nature of cortical slow waves

doi: 10.1016/j.isci.2025.113213

Figure Lengend Snippet: SWA propagation axis are controlled by excitable populations in vivo (A) Schematic representation of the in vivo recording setup. (B) Representative example of the SWA activity recorded. Each color represents and individual shank, placed as shown in (A). Shaded traces were recorded in the cortex and used for further analysis. (C) Example of the detection of Up states, shown as faint red bars, based on the calculation of the log MUA of the recorded trace, shown in orange and blue respectively. (D) Relative delay of each shank along the Up states of one recordings session. Negative values are shown in the shanks on which the Up state was recorded first. Note how after NE + Cch are released, the more caudal shank (orange) shifts from positive to negative delays, while the contrary occurs for the rest of the shanks, as the wave becomes predominantly caudorostral. (E) Time lag matrix showing all the cortical electrodes and Up states recorded in a session. Note how following the release the caudal shank (orange) shifts from positive to negative delays, meaning that it becomes the first shank on which the Ups are detected. (F) Change in the ratio of rostrocaudal Up states following the release in all the recorded sessions. There is a significant ( p = 0.039) decrease in the amount of rostrocaudal Up states. Control recordings, on which saline was released as a substitute of the NE + CCh cocktail, do not show a significant change in the directionality of the SWA. p values calculated using Wilcoxon signed-rank test ( p value <0.05).

Article Snippet: One-third of the original induction dose was injected intramuscularly to maintain anesthesia once paw reflex could be evoked or the LFP trace exhibited signs of awakening, approximately every 2 h. Extracellular activity was then recorded by two linear silicon probe electrodes (NeuroNexus Technologies, Ann Arbor, MI).

Techniques: In Vivo, Activity Assay, Control, Saline